Supplementary Figure 2.

E-cadherin peptides do not significantly affect Caco-2 proliferation or apoptosis. Caco-2 cells were grown on plastic and scratch-wounded and stained with EdU as a marker of cell proliferation (green), terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick-end labeling (TUNEL) stain for apoptotic cells (red), and 4′,6-diamidino-2-phenylindole to show nuclei (blue) under (A and B) serum-free and (C and D) 10% serum conditions. Representative images (A and C) of wounded monolayers and summary data (B and D) for EdU staining and TUNEL staining at 48 hours. E-cadherin peptides had no effect on either parameter. Similar results were seen at 30-minute and 24-hour treatment times (data not shown). *P < .05, ***P < .001 (using a 1-way analysis of variance with the Dunn post-test). N = 3 independent experiments, each with 3 or more technical replicates.