Supplementary Table 1.
Sequences and Characteristics of Peptide Fragments of Human E-Cadherin Generated by NE In Vitro
| Peptide name | Peptide sequence | Position | Domain |
|---|---|---|---|
| EcadP-1 | FDYEGSGSEAA | 833-843 | α β γ catenin binding domain |
| EcadP-2 | TVTDQNDNKPEFT | 251-263 | Between cadherin domains 1 and 2 (calcium binding) |
| EcadP-3 | QAADLQGEGLSTTAT | 346-360 | Cadherin domain 2 |
| EcadP-4 | SSNGNAVEDPMEI | 236-248 | Cadherin domain 1 |
| EcadP-5 | NNDGILKTA | 431-439 | Cadherin domain 3 |
| EcadP-6 | QYNDPTQESI | 641-650 | Cadherin domain 5 |
| EcadP-7 | SLTTSTATV | 465-473 | Cadherin domain 3 |
| EcadP-8 | SEDFGVGQEI | 496-505 | Cadherin domain 4 |
| EcadP-9 | TNPVNNDGILKT | 427-438 | Cadherin domain 3 |
| EcadP-10 | ATDADDDVNTYNAAI | 286-300 | Cadherin domain 2 (calcium binding) |
| EcadP-11 | TTNPVNNDGILKTA | 426-439 | Cadherin domain 3 |
| EcadP-12 | LNDDGGQFVV | 417-425 | Cadherin domain 3 |
| EcadP-13 | TYKGQVPENEANV | 378-391 | Cadherin domain 3 (calcium binding) |
| EcadP-14 | RNDVAPTLM | 784-792 | Cytoplasmic |
| EcadP-15 | KAADTDPTAPPYD | 816-828 | α β γ catenin binding domain |
| EcadP-16 | SGIQAELL | 133-140 | Propeptide |
| EcadP-17 | LPPEDDTRDNV | 741-752 | Cytoplasmic |
| EcadP-18 | TGQGADTPPVGV | 193-204 | Cadherin domain 1 |
| EcadP-19 | LLILILLLLL | 721-729 | Cytoplasmic |
| EcadP-20 | ALIIATDNGSPV | 563-574 | Cadherin domain 4 |
| EcadP-21 | EAGLQIPA | 702-709 | Ectodomain/transmembrane junction |
| EcadP-22 | NRNTGVISVV | 320-329 | Cadherin domain 2 |
| EcadP-23 | TVTDTNDNPPIFNPT | 364-377 | Between cadherin domains 2 and 3 (calcium binding) |
| EcadP-24 | NNDGILKT | 431-438 | Cadherin domain 3 |
NOTE. Samples were sequenced by mass spectroscopy to show 24 distinct peptides. Sequences are shown, as well as the regions of the full-length E-cadherin that they represent. Ecad, E-cadherin.