Supplementary Figure 1.

Formation of isoLG protein adducts in vitro. (A) EPC-2 cells were treated with BA/A for 15 minutes and analyzed for isoLG protein adducts with D11 antibody. Treatment with BA/A led to significant accumulation of isoLG adducts in EPC-2 cells. D11 positivity was arbitrarily set at 1 in control samples. (B) The same as A, but CP-A cells are shown. (C) Representative mass spectrometry chromatograms show levels of isoLG-lysine lactam adducts in cellular extracts collected from control (untreated) and BA/A-treated TE-7 cells in the presence or absence of 2-HOBA. Statistical analyses were performed with the Student t test. *P < .05, **P < .01. Data are represented as mean ± standard deviation.