Supplementary Figure 3.

Acidic bile salts cause the formation of adducts on p53 protein and its precipitation. (A) p53 protein is precipitated after treatment of EPC-2 cells with BA/A. 2-HOBA prevents precipitation of p53 protein. (B) The same as A, but HET-1A cells are shown. (C) Analysis of isoLG protein adducts using D11 scFv after immunoprecipitation of p53 protein with p53-specific antibody (DO1) in EPC-2 cells. Exposure of esophageal cells to BA/A significantly increases levels of p53 protein adducts. Each analysis is representative of 3 independent experiments and values are expressed as mean ± standard deviation. D11 positivity was arbitrarily set at 1 in control samples. Statistical analyses were performed with the Student t test. *P < .05, **P < .01.