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. Author manuscript; available in PMC: 2019 Mar 11.
Published in final edited form as: Anal Chem. 2018 Sep 28;90(20):11735–11740. doi: 10.1021/acs.analchem.8b01405

Figure 2. Applying DRONE to track APOBEC3G enzyme turnpover.

Figure 2

A. Development of a scheme to purify enzyme timepoint experiments by UHPLC analysis. B. A schematic depicting cytosine to uracil conversion catalyzed by recombinant APOBEC3G C. Conversion of substrate to product catalyzed by recombinant APOBEC3G over the course of 2.5 hours. Reaction was conducted under single turnover conditions at 5 μM recombinant APOBEC3G and 1 μM substrate oligonucleotide. Quantitation based on AUC analysis is shown on the bottom right representing mean +/− SEM of % product values based on three biological replicates per timepoint.