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. 2019 Feb 25;2019:4084351. doi: 10.1155/2019/4084351

Figure 4.

Figure 4

EPN1 and EPN2 silencing affects neuroepithelial cells polarization and neural rosette lumen size. (a) Nestin immunofluorescence analysis reveals impaired neuroectoderm polarization and neural rosette lumen size in 7 day-neuralized EPN1 and EPN2 KD 46C mESC cultures. (b, c) Immunofluorescence analysis and relative size quantification of N-cadherin+ve lumens show that EPN1 KD and EPN2 KD induce a decrease in the percentage of large-lumen rosettes. (d) After 11 days of exposure to neuralizing conditions, EPN1 and EPN2 KD mESC cultures show an increase in βIII-tubulin+ve and MAP 2+ve neurons as scored by immunofluorescence analysis. Nuclei are counterstained with Hoechst 33258. All data are expressed as the means ± STDV ((a, d): n = 3 biologically independent experiments; (b, c): n = 5 biologically independent experiments). Statistical significance (unpaired t-test): ∗ p < 0.05.