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. 2019 Feb 25;2019:8639791. doi: 10.1155/2019/8639791

Figure 1.

Figure 1

MnTE-2-PyP inhibits TGF-β-induced morphological changes in colorectal cancer cells. (a) The morphological changes of LOVO, HT29, and SW480 cells after exposure to MnTE-2-PyP alone, TGF-β alone, or MnTE-2-PyP combined with TGF-β were evaluated using light microscopy (×400 magnification). The cells were pretreated with MnTE-2-PyP (30 μM) for 12 h and then treated with TGF-β (5 ng/ml) for two days. The cells lost their polarized epithelial phenotype with increased cell-cell connections and acquired mesenchymal traits after two days of TGF-β treatment. The cells became dispersed and assumed a fibroblast-like appearance. MnTE-2-PyP treatment inhibited TGF-β-induced morphologic changes significantly in LOVO, HT29, and SW480 cells. After treatment with MnTE-2-PyP, these cells kept a more epithelial-like appearance induced by TGF-β.