Table 1.
Cultivation parameters of S. cerevisiae ST938 grown in continuous cultures under C-limited conditions
| Chemostat | Batch | ||||
|---|---|---|---|---|---|
| Dilution rate (h−1) | µmax (h−1) | ||||
| 0.04 | 0.09 | 0.17 | 0.21 | 0.27 | |
| YX/S (g mol−1) | 78.4 ± 0.6 | 79.7 ± 5.8 | 90.3 ± 6.6 | 102.4 ± 0.7 | 24.9 ± 1.3 |
| YEtOH/S (mol mol−1) | 0.09 ± 0.03 | 0.04 ± 0.02 | 0.04 ± 0.01 | 0.04 ± 0.00 | 1.1 ± 0.0 |
| YGly/S (mol mol−1) | 0.03 ± 0.01 | 0.02 ± 0.00 | 0.02 ± 0.00 | 0.00 ± 0.00 | 0.1 ± 0.0 |
| Y3-HP/S (mol mol−1) | 0.33 ± 0.03 | 0.30 ± 0.00 | 0.26 ± 0.00 | 0.23 ± 0.00 | 0.01 ± 0.00 |
| Y3-HP/S (%C-mol) | 16.6 ± 1.7 | 15.1 ± 0.4 | 13.1 ± 0.4 | 11.7 ± 1.5 | 0.6 ± 0.0 |
| Y3-HP/X (g gCDW−1) | 0.43 ± 0.04 | 0.34 ± 0.03 | 0.26 ± 0.01 | 0.19 ± 0.02 | 0.04 ± 0.00 |
| qS (mmol gCDW−1 h−1) | 0.51 ± 0.13 | 1.2 ± 0.1 | 1.9 ± 0.2 | 2.1 ± 0.0 | 10.7 ± 0.7 |
| qGly (mmol gCDW−1 h−1) | 0.01 ± 0.00 | 0.02 ± 0.00 | 0.03 ± 0.00 | 0.06 ± 0.01 | 0.86 ± 0.03 |
| qEtOH (mmol gCDW−1 h−1) | 0.05 ± 0.02 | 0.05 ± 0.02 | 0.07 ± 0.02 | 0.08 ± 0.01 | 11.9 ± 0.5 |
| q3-HP (mmol gCDW−1 h−1) | 0.17 ± 0.06 | 0.36 ± 0.03 | 0.50 ± 0.03 | 0.49 ± 0.07 | 0.12 ± 0.00 |
| Glcres (mM) | 0.07 ± 0.00 | 0.08 ± 0.01 | 0.06 ± 0.03 | 0.05 ± 0.02 | n.a. |
Errors correspond to standard deviations derived from triplicate cultivations
Y yield, q rate, X biomass, S substrate (glucose), EtOH ethanol, Gly glycerin, 3-HP 3-hydroxypropionic acid, Glcres residual glucose, n.a. not applicable