Table 2.
Cultivation parameters of S. cerevisiae ST938 grown in continuous cultures at a dilution rate of 0.04 h−1 under carbon (C), nitrogen (N) and phosphorus (P) limited conditions
Chemostat [0.04 h−1] | |||
---|---|---|---|
C-limitation | N-limitation | P-limitation | |
YX/S (g mol−1) | 78.4 ± 0.6 | 72.4 ± 0.5 | 75.2 ± 1.9 |
YEtOH/S (mol mol−1) | 0.09 ± 0.03 | 0.05 ± 0.01 | 0.04 ± 0.01 |
YGly/S (mol mol−1) | 0.03 ± 0.01 | 0.09 ± 0.01 | 0.01 ± 0.00 |
Y3-HP/S (mol mol−1) | 0.33 ± 0.03 | 0.36 ± 0.04 | 0.42 ± 0.01 |
Y3-HP/S (%C-mol) | 16.6 ± 1.7 | 17.7 ± 1.9 | 21.1 ± 1.8 |
Y3-HP/X (g gCDW−1) | 0.43 ± 0.04 | 0.44 ± 0.05 | 0.50 ± 0.01 |
qS (mmol gCDW−1 h−1) | 0.51 ± 0.13 | 0.61 ± 0.01 | 0.54 ± 0.01 |
qGly (mmol gCDW−1 h−1) | 0.01 ± 0.00 | 0.06 ± 0.01 | 0.01 ± 0.00 |
qEtOH (mmol gCDW−1 h−1) | 0.05 ± 0.02 | 0.03 ± 0.01 | 0.02 ± 0.01 |
q3-HP (mmol gCDW−1 h−1) | 0.17 ± 0.06 | 0.22 ± 0.02 | 0.23 ± 0.01 |
Glcres (mM) | 0.07 ± 0.00 | 0.63 ± 0.13 | 0.19 ± 0.03 |
PO4res (mM) | n.a. | n.a. | b.d. |
NH4res (mM) | n.a. | b.d. | n.a. |
Errors correspond to the standard deviations derived from triplicate cultivations. The C-limitation data were taken from Table 1 for simplicity of comparison
Y yield, q rate, X biomass, S substrate (glucose), EtOH ethanol, Gly glycerin, 3-HP 3-hydroxypropionic acid, Glcres residual glucose, PO4res residual phosphate, NH4res residual ammonium, n.a. not applicable, n.d. not determined, b.d. below detection limit