Table 3.
Cultivation parameters of S. cerevisiae ST938 grown in fed-batch mode in 1-L bench-top bioreactors under carbon (C) and phosphorus (P) limiting conditions
| Fed-batch | ||
|---|---|---|
| C-limitation | P-limitation | |
| M (h−1) | 0.059 | 0.051 |
| YX/S (g mol−1) | 77.9 ± 8.9 | 67.7 ± 6.4 |
| YEtOH/S (mol mol−1) | b.d. | b.d. |
| YGly/S (mol mol−1) | b.d. | b.d. |
| Y3-HP/S (%C-mol) | 15.9 ± 1.2 | 25.6 ± 1.7 |
| Y3-HP/X (g gCDW−1) | 0.38 ± 0.02 | 0.65 ± 0.09 |
| qS (mmol gCDW−1 h−1) | 0.75 ± 0.10 | 0.77 ± 0.05 |
| q3-HP (mmol gCDW−1 h−1) | 0.24 ± 0.00 | 0.39 ± 0.01 |
| qCO2 (mmol gCDW−1 h−1) | 1.94 ± 0.48 | 1.88 ± 0.16 |
| Carbon balance (%) | 105 ± 4.3 | 100 ± 4.2 |
The growth rate (µset) of the exponential feeding profile was 0.05 h−1. Errors correspond to the standard deviations derived from triplicate cultivations
X biomass, S substrate (glucose), EtOH ethanol, Gly glycerin, 3-HP 3-hydroxypropionic acid, b.d. below detection limit