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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Nat Neurosci. 2019 Feb 4;22(3):374–385. doi: 10.1038/s41593-018-0334-7

Fig. 5 |. C4 SZ risk variants increase complement deposition on neurons and increase synapse engulfment in in vitro models derived from SZ patients.

Fig. 5 |

a, Significant correlation between neural C4AL CNs and neuronal C3 complement deposition; no effect of C4BL (b) or C4BS (c) CN on C3 deposition was observed. n = 13 SZ patients (means from 36 fields per well and 8 wells per line for each experimental condition. d, Representative confocal images froma,b of C3 deposition after IgM anti-NCAM antibody alone (negative control, top row), with the addition of C1, C2, and C3 (second row) across lines with increasing C4AL CNs and decreasing C4B CNs, and with the addition of C5-depleted serum (positive control, bottom row). Scale bar, 100 μ m. e, Similar correlation as in a but between C4AL CNs (SZ patients also described in Fig. 3c; n = 19 patients) and SYN uptake as measured by PSD-95+ phagocytic inclusions. f, Representative confocal images for the two groups. Scale bar, 30 μ m. All reported P values are two-sided; correlation coefficients are Spearman’s.