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Identification of the liposome-binding region using Ambn Trp variants. Trp fluorescence spectra of (A) 2.0 μM AmbnW80F/W93F and (B) AmbnW402F variants titrated with 300 μM LUVs. The insets show the emission maxima of the Trp fluorescence spectra. Student’s t-test, n = 3.
