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Formation of AH is vital for liposome-binding ability. (A–E) Trp fluorescence spectra of 10.0 μM peptide variants titrated with 300 μM LUVs. (F) Blue shifts of the emission maxima of the Trp fluorescence spectra of the peptide variants shown in A to E (n = 2). (G–J) CD spectra of 10.0 μM peptide variants titrated with 300 μM LUVs. (K,L) Normalized membrane leakage of LUVs in the presence of 1.0 μM peptide variants R69D/K74D, K74D, K74L, N77K, and F72S.
