Figure 4.

Transcriptomic analysis of pyrvinium treatment. LAPC4 cells were treated overnight with vehicle, 0.3 nM DHT, or DHT +100 nM PP. RNA was harvested and sequenced. Principal component analysis (A) demonstrates clustering of biological duplicates, as does hierarchical clustering (B). Gene ontology demonstrates appropriate response to DHT (C). (D) PP treatment caused decreased nuclear hormone receptor signaling as well as P53 and ERBB2 signaling; it also caused activation of pathways unrelated to nuclear hormone signaling.