Figure 5.

Effect of pyrvinium on alternative splicing. (A) Gene ontology analysis of transcripts with differential exon inclusion with PP treatment demonstrated that most are involved in alternative splicing and RNA processing. qRT-PCR analysis of (B) SRSF3 or (C) SRSF7 transcript levels. DHT treatment reduces the total levels of mature transcripts (constitutive) as well as the levels of PTC-containing transcripts, which are further reduced in a dose-dependent fashion by PP treatment. (D) qRT-PCR analysis of AR exons 1 and 7 (with the ratio of exon1/exon7 shown in the inset) and ARV-7. * significantly different compared to DHT treatment with (p < 0.05).