Table 2. Negative binomial regression modela for the rate of inactivation of E. coli O157:H7b on heads of romaine lettuce (n = 559) over 10 days in central coastal California during July–Aug. and Oct., 2012.
| Factor | Factor Categories | Coefficient | Std. Err. | 95% C.I. | P-value |
|---|---|---|---|---|---|
| Fecal | Pigc | 0.0 | |||
| Rabbit | −1.232 | 0.334 | −1.888, −0.576 | 0.000 | |
| Chicken | −1.289 | 0.342 | −1.959, −0.619 | 0.000 | |
| Trial | Julyc | 0.0 | |||
| October | −4.066 | 0.375 | −4.801, −3.331 | 0.000 | |
| Trial × Fecal | July × Pigc | 0.0 | |||
| October × Rabbit | 1.929 | 0.487 | 0.974, 2.884 | 0.000 | |
| October × Chicken | 2.016 | 0.480 | 1.076, 2.956 | 0.000 | |
| Daily leaf wetnessd | 0.041 | 0.012 | 0.017, 0.065 | 0.001 | |
| Sampling evente | 1 (0.5 ± 0.3)c | 0.0 | |||
| 2 (21.8 ± 0.8) | −0.657 | 0.391 | −1.423, 0.109 | 0.093 | |
| 3 (44.6 ± 0.1) | −1.565 | 0.490 | −2.526, −0.605 | 0.001 | |
| 4 (68.9 ± 0.4) | −4.170 | 0.408 | −4.97, −3.371 | 0.000 | |
| 5 (93.2 ± 0.5) | −5.642 | 0.408 | −6.441, −4.842 | 0.000 | |
| 6 (115.9 ± 0.1) | −3.880 | 0.450 | −4.762, −2.998 | 0.000 | |
| 7 (119.5 Oct.)f | −6.167 | 0.517 | −7.179, −5.154 | 0.000 | |
| 7 (123.0 July)f | −0.133 | 0.655 | −1.417, 1.150 | 0.839 | |
| 8 (141.7 ± 0.5) | 3.215 | 0.462 | 2.308, 4.121 | 0.000 | |
| 9 (164.9 ± 0.4) | −5.130 | 0.426 | −5.965, −4.295 | 0.000 | |
| 10 (188.5 ± 0.5) | −7.180 | 0.419 | −8.002, −6.359 | 0.000 | |
| 11 (212.8 ± 0.3) | −6.274 | 0.462 | −7.179, −5.369 | 0.000 | |
| 12 (235.4 ± 0.6) | −9.230 | 0.396 | −10.005, −8.454 | 0.000 | |
| Intercept | 2.997 | 0.754 | 1.520, 4.474 | 0.000 |
Notes:
Regressed onto Ct as the outcome variable.
Bacteria concentrations were estimated using MPN methods.
Referent.
Based on the averaged HOBO data collected in 15-min. intervals beginning 24 h prior to harvest.
Categorized time since inoculation with actual hours displayed in parenthesis.
Event 7 was modeled as two separate events to capture the 4.5 h collection lag between both trials.