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. 2019 Mar 8;7:e6589. doi: 10.7717/peerj.6589

Figure 2. Osteogenic differentiation of PDLCs and hMSCs after 14 days of osteo-inductive incubation in vitro.

Figure 2

(A) ALP staining. The PDLCs and hMSCs kept in normal growth medium were used as control, while the PDLCs and hMSCs kept in osteo-inductive medium were assessed as test groups. (B) ALP activity assay. (C) von Kossa staining of PDLCs and hMSCs after 14 days of osteo-inductive incubation. (D) Quantitative analysis of von Kossa staining. (E) Alizarin red S staining of PDLCs and hMSCs after 14 days of osteo-inductive incubation. (F) Quantitative analysis of Alizarin red S staining. Scale bars, 100 μm. (Student’s t-test, n ≥ 3; *P < 0.05, **P < 0.01).