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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Oral Oncol. 2019 Feb 1;90:38–44. doi: 10.1016/j.oraloncology.2019.01.017

Figure 3 – Ionizing radiation variably enhances direct haNK cytolysis of HNSCC cell lines.

Figure 3 –

(A) HNSCC cell lines were plated with and without prior exposure to 8Gy IR were plated and allowed to gain impedance overnight prior to the addition of haNKs at the indicated E:T ratios. Cell index plots normalized to the addition of haNKs at time 0. Percent loss of cell index relative to control (no haNKs) 24 hours after the addition of haNKs quantified to the right of each cell index plot. (B) HNSCC cells were assessed for changes in inhibitory and activating ligands for NK function 24 hours after a single dose of 8Gy IR by flow cytometry. Representative results from one of at least two independent assays shown, each performed in at least technical triplicate. *, p<0.05; **, p<0.01; ***, p<0.001; student’s t-test or ANOVA.