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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Dev Biol. 2018 Dec 13;446(1):102–118. doi: 10.1016/j.ydbio.2018.12.014

Figure 1: Live-imaging reveals the stages of hub formation. A – C).

Figure 1:

Three stages of niche formation: Gonad Coalescence, Hub Assembly, and Hub Compaction are shown using a selected still from live-imaging of six4GFPnls (green), which marks all SGPs. A) A view of a coalesced gonad, imaged in vivo at 12h AEL, showing that SGPs were relatively dispersed, intermingled with germ cells (large negatively-marked spaces). B) A view of a gonad at Hub Assembly, imaged in vivo at 16h AEL, showing that a subset of SGPs had assembled at the anterior forming the prospective hub (left of dotted line), and had recruited a tier of prospecive germline stem cells (negative space; GSCs). C) A view of a gonad after Hub Compaction, imaged ex vivo at 22 h AEL. D, E) For each of the three stages, two schematics, each at an increasing level of detail, are drawn directly below their respective live image view. The schematics are labeled using the same terms as in the text, and a key is shown along the right hand side. All SGPs, green; msSGPs, bright green. In E), in addition to all SGPs (green), this series marks PS 11 cells and msSGPs (red). Some PS11 cells will assemble with PS10 cells as a pro-niche. Scale bar is 10 microns.