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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Dev Biol. 2018 Dec 13;446(1):102–118. doi: 10.1016/j.ydbio.2018.12.014

Figure 4: The hub often adopts an internal tilt, relative to A-P axis of embryo.

Figure 4:

A, B, C)The final time point from a movie visualizing niche assembly (six4-nlsGFP). Each sequence shows Z slices starting from the external region of the gonad, and moving more internally, with the fractional position along the external-internal gonad axis indicated (0, most external; 1, most internal). Niche cells (arrow), msSGPs (asterisk), and either trachea (A, C; breathless>GFP, blue arrowhead), or the Alary (C; tupAME-GFP, blue arrowhead). Scale bar is 10 microns. D) Frequency distribution of the tilt of the niche. E) Schematic of an embryo showing a dorsal view of the gut, the gonads, and an internally tilted niche in each gonad.