KEY RESOURCES TABLE
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti Vasa | R. Lehmann | N/A |
| Goat polyclonal anti Vasa | Santa Cruz | Cat#dC-13 (discontinued) |
| Rabbit polyclonal anti STAT92E | E. Bach | N/A |
| Rabbit polyclonal anti RFP | Abcam | Cat#ab62341 |
| Mouse monoclonal anti Fasciclin III | Developmental Studies Hybridoma Bank (DSHB) | Cat#7G10 |
| Mouse monoclonal anti Islet | DSHB | Cat#40.3A4 |
| Mouse monoclonal anti Crumbs | DSHB | Cat#cq4 |
| Rat monoclonal anti DE-cadherin | DSHB | Cat#DCAD2 |
| Guinea pig polyclonal anti Traffic jam | D. Godt | N/A |
| Chick polyclonal anti GFP | Aves Labs | Cat#GFP-1020 |
| Normal Donkey Serum (NDS) | Jackson Immunoresearch Laboratories | 017-000-121 |
| Alexafluor Secondary Antibodies (488, 647) | Molecular Probes | |
| Cy3 Affinipure Secondary Antibodies | Jackson ImmunoResearch | |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| s700 halocarbon oil | Sigma | Cat#H8898 |
| Poly Lysine | Sigma | Cat#P-1274 |
| FBS | GIBCO | Cat#10082 |
| penicillin/streptomycin | Corning | Cat#30-002-Cl |
| Schneider’s Insect Media | GIBCO | Cat#21720-024 |
| Insulin,Bovine | Sigma | Cat#l0516 |
| Formaldehyde, 16% | Electron Microscopy Sciences | Cat#15710 |
| Formaldehyde, 40% | Electron Microscopy Sciences | Cat#15715-S |
| Hoechst | Sigma | Cat#33342 |
| Phosphatase Inhibitor | Sigma | Cat#04906837001 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: six4-nls-eGFP | D. Finnegan | Clark et al, 2006 |
| D. melanogaster: P-Dsix4-eGFP::Moesin | R. Lehmann | Sano et la 2012 |
| D. melanogaster: nos-moesin::GFP | R. Lehmann | Sano et al 2005; FBtp0040584 |
| D. melanogaster: sqh-Sqh::mCherry | A. Martin | Mason et al, 2013 |
| D. melanogaster: tupAME-moeGFP | M. Frasch | Boukhatmi et al 2014 |
| D. melanogaster: TypeIV Collagen::GFP | Kyoto Stock Center (KSC)110692 | FBti0153267 |
| D. melanogaster: His2Av::mRFP1 | Bloomington Drosophila Stock Center (BDSC) | FBtp0056035 |
| D. melanogaster: Perlecan::GFP | trolZCL1700, KSC 110807 | FBti0129820 |
| D. melanogaster: abdA-Gal4 | Merabet, Marseille | Hudry et al, 2011 |
| D. melanogaster: Prd-Gal4 | BDSC | FBst0001947 |
| D. melanogaster: tup-Gal4 | BDSC | FBst0046960 |
| D. melanogaster: tupAME-Gal4 | J.-L. Frendo | N/A |
| D. melanogaster: breathless-GAL4 | BDSC | FBst0078328 |
| D. melanogaster: UAS-tdTomato | BDSC | FBst0036328 |
| D. melanogaster: P{UAS-grim.Y}2 | H. Bellen | FBtp0009995 |
| D. melanogaster: mys1 / FM4 | BDSC | FBst0000059 |
| D. melanogaster: trh8 / TM3, twi-Gal4, UAS-GFP | D. Andrew | FBal0050667 |
| D. melanogaster: y1 w*; tup1 P{neoFRT}40A / CyO | BDSC | FBst0036503 |
| D. melanogaster: tupex4 | S. Campuzano | FBal0216723 |
| D. melanogaster: CyO, P{w[+mC]=ActGFP}JMR1 | BDSC | FBst0004533 |
| D. melanogaster: TM3, P{w[+mC]=GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb[1] Ser[1] | BDSC | FBst0006663 |
| Oligonucleotides | ||
| GAA GAA TCC CAG CAA AGA CCG TGA GTT G | Clark et al, 2006 | D-six4 third intron, forward, EcooRI site |
| GTT GGA TCC ATT GCC ATC CAG TTG | Clark et al, 2006 | D-six4 third intron, reverse, BamH1 site |
| Recombinant DNA | ||
| H-GAL4 vector | S. Barolo | Castro et al, 2005; 10.1242/dev.01920 |
| Software and Algorithms | ||
| FIJI (Image J) | www.fiji.sc | N/A |
| Image J | www.imagej.nih.gov/ij/ | N/A |
| Metamorph Microscopy Automation and Image Analysis Software | Leica | v7.8.40 |
| Axio-Vision Imaging Software | Zeiss | v4.8.1 |
| Graphpad Prism | Graphpad Software | v7.0 |
| Other | ||
| Matek imaging dish | Thermofisher | P35G-1.5-14-C |