Fig. 5.

Conservation of local frustration and sequence for the aldolases family. (A) FrstIC based on the single-residue level frustration index. Green, minimally frustrated contacts; red, highly frustrated contacts; gray, neutral contacts. Black circles show the values of the SeqIC. (B) Aldolase structure: in green and red, residues with FrstIC values greater than 0.5, minimally frustrated and highly frustrated, respectively. (C) Catalytic residues at the active site. (D) Frustration index at the contact level. Red lines correspond to highly frustrated interactions of the catalytic residues (in yellow) among themselves and with nearby residues in the structure.