Fig. 1.

Assessment of human haploid leukemia (HAP1) cells deficient in TRPM7. (A) Determination of elementary levels of Ca (Left), Mg (Center), and Zn (Right) in wild-type (WT) and TRPM7-deficient (KO) HAP1 cells. Dried cell pellets were obtained from WT and KO HAP1 cells cultured in a standard cell culture medium for 24 h and analyzed by ICP-MS. Total elementary contents were normalized to dry pellet weight and represented as mean ± SEM of n = 4 independent measurements. (B) Total elementary contents of divalent metals obtained in A were normalized to total elementary contents of sulfur (S) and represented as mean ± SEM. ***P ≤ 0.001; **P ≤ 0.01; n.s., not significant (Student’s t test). (C) Uptake of ⁶⁵Zn²⁺ in WT and KO HAP1 cells. Cells were incubated in the presence of 1 mM Mg²⁺, 2 mM Ca²⁺, and 2 µM ⁶⁵Zn²⁺ and time-dependent accumulation of ⁶⁵Zn²⁺ was determined, presented as mean ± SEM for n = 6 independent measurements. Datasets were fitted using a one-phase exponential association equation followed by a statistical assessment with the extra sum-of-squares F test.