Fig. 3.

Expression of (A) hCG, (B) P4, (C) PGF, and (D) sFLT-1 in 24-h culture between day 5 and day 6 following initiation of BAP-induced differentiation. The experiment used two ESCs (H1, H9*; *female line), four CTL TB (5*, 6, 7, 8*), and four EOPE TB (A*, J, M, R*) lines. The concentrations of the various proteins measured by ELISA were normalized to DNA content of the cultures. Values are means ± SE for three individual experiments. Pairwise comparisons were determined by using the Student t test, with significance (P < 0.05) shown by asterisks.