Fig. 4.

PCA of RNAseq data from CTL TB and EOPE TB (GSE119265). Data are based on the log-transformed TPM from the top quartile of the most variable genes. (A) Each dot represents an individual sample from either CTL TB 5% O₂ (red), CTL TB 20% (black), EOPE TB 5% (blue), or EOPE TB 20% (green). The first three principal components show a separation by O₂ treatment but not by disease (CTL vs. EOPE). (B) The same analysis as in A but showing the sex of each line. Each dot represents an individual sample from either female CTL TB 5% O₂ (green), female CTL TB 20% (black), male CTL TB 5% O2 (blue), male CTL TB 20% (red); female EOPE TB 5% (yellow), female EOPE TB 20% (cyan), male EOPE TB 5% (gray), or male EOPE TB 20% (magenta). Shown on both plots are the locations of the CTL cultures derived from patients 7 and 8 (Table 1), where the infant was born prematurely, and EOPE cultures E and S, which represent their nearest neighbors in the PCA plot. Also shown are locations of two male EOPE lines, J and I, derived from UC of dichorionic twins (Table 1).