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. 2019 Feb 21;116(10):4548–4557. doi: 10.1073/pnas.1813802116

Fig. 5.

Fig. 5.

FZD3 mediates invasive and metastatic properties of melanoma. (A) FZD3 knockdown leads to the significant down-regulation of the genes associated with melanoma progression and metastasis. Heatmap analysis of RNA-Seq data using two biological replicate experiments analyzing M727 cells transduced with shSC and shFZD3. (B and C) Matrigel cell invasion assay of M727 cells transduced with shSC or shFZD3 lentivectors. (Scale bars, 200 μm.) Error bars represent SEM of duplicate experiments with two replicates each. (D) Representative images of human melanoma Ku-80 nuclear antigen immune-fluorescence staining of kidneys and (G) lungs tissue sections from mice transplanted with indicated M727 cells. Green signal indicates positive Ku-80 signal and marks the presence of human melanoma cells throughout the mouse tissue. (Scale bars, 1,000 μm.) Average number of metastatic lesions per (E) kidney and (H) lungs (n = 5 mice). Estimated number of metastatic melanoma cells found per (F) kidney and (I) lung of transplanted mice using human Alu real-time PCR approach. Error bars represent SEM of triplicate experiments with two replicates each.