Fig. 3.
Podxl promotes endothelial cell adhesion and barrier function. ECIS ZΘ assay of barrier function of control (scrambled siRNA; black line) and PODXL knockdown (PODXLKD; gray line) HUVECs. Cell-free wells (dashed line) served as a negative control. Cells were seeded on matrix-coated 8W10E+ PET wells at a density of 1.5 × 105 cells/well. Electrode coverage and barrier parameters were measured at multiple frequencies (f). (A) Electrode coverage was measured as capacitance (nF) at 64 kHz. (B) Barrier formation was measured as resistance (W) at 4 kHz. (C) Modeling of cell–cell interactions (Rb). (D) Modeling of cell–matrix interactions (α). ***Significantly different from control with P < 0.0001 (determined by Student’s t test) (n = 3 per condition).