Fig. 1.
Cryo-EM structure of NCLP6A and NCLP5′. (A and B) Representative cryo-EM micrographs of NCLP6A (A) and NCLP5′ (B), with a representative class average shown as Inset. (Scale bar: 20 nm.) (C and D) Isosurface representation of the cryo-EM maps of NCLP6A (C) and NCLP5′ (D) colored as in the previously published work (11): NTDARM in blue navy, NTD in blue, CTD in salmon, CTDARM in yellow, RNA in green. A front view and a cutaway view are shown. (E and F) Zoom of three consecutive protomers of NCLP6A (E) and NCLP5′ (F) viewed from the helix exterior. Each RNA molecule is colored in different shades of green and the gaps between the RNA molecules are highlighted by red arrows. (G and H) Zoom of protein–RNA interaction in NCLP6A (G) and NCLP5′ (H), with asterisks to highlight gaps between the RNA molecules. Some clearly visible side chains described in the text are indicated.