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Changes in the active site during the ATPase cycle of polymerized actin. (A and B) AMPPNP-actin. (C) ADP-Pi-actin. (D) ADP-actin. (A) A small ribbon diagram looking down into the active site for orientation. (B–D) Ribbon diagrams with stick figures and map densities zoned within 2.12 Å of the nucleotide and the important side chains D11, K18, Q137, D154, and H161.
