Fig. 1.
Miniaturization and parallelization of droplet dELISA. (A) A schematic of the conventional workflow for dELISA, which requires multiple hands-on steps and is rate-limited by the serial partitioning of the sample into droplets and the serial detection of the fluorescence of each individual droplet. (B) The MD parallelizes droplet generation, incubation, and detection to miniaturize dELISA fully onto a mobile platform and increase its throughput by 100×. (C) Antibody-functionalized, color-coded beads are used in a duplex dELISA assay, wherein individual beads are encapsulated into droplets and read out if they have captured a single target protein.