Table 2. Quantitative comparison of fluorescent signal level between P-IMS and HS-IMS systems. Three arbitrary selected microspheres (FluoSpheres, #F8841, Thermo-Fisher) were imaged at 30, 20, 10 and 5 ms exposure times respectively. Averaged (168 images) intensity values integrated over spatial and spectral voxels of selected beads for both systems are given in columns ItotHS-IMS and ItotM-IMS. Count of photo-electrons recorded by HS-IMS and P-IMS systems over spatio-spectral voxels of each bead is given in Ie-HS-IMS and Ie-M-IMS respectively. Please note that signal recorded by P-IMS was multiplied by 0.5 to account for 2x binning in y direction.
| Exposure time [ms] | ItotHS-IMS [a.u.] | Ie-HS-IMS [e-] | ItotM-IMS [a.u.] | Ie-M-IMS[e-] | Ie-HS-IMS/Ie-M-IMS | |
|---|---|---|---|---|---|---|
| Bead #1 | 30 | 1371800 | 664170 | 154040 | 139660 | 4.7 |
| 20 | 908570 | 457920 | 107000 | 97018 | 4.7 | |
| 10 | 464090 | 233900 | 51466 | 46664 | 5.0 | |
| 5 | 231210 | 116530 | 23784 | 21565 | 5.4 | |
| Bead #2 | 30 | 1696400 | 854990 | 190380 | 172620 | 4.9 |
| 20 | 1175200 | 592300 | 131220 | 118980 | 5.0 | |
| 10 | 594710 | 299730 | 62994 | 57117 | 5.4 | |
| 5 | 273870 | 138030 | 28272 | 25634 | 5.2 | |
| Bead #3 | 30 | 1193700 | 601620 | 149070 | 135160 | 4.4 |
| 20 | 789850 | 398080 | 103090 | 93472 | 4.3 | |
| 10 | 413270 | 208290 | 50468 | 45759 | 4.5 | |
| 5 | 194050 | 97801 | 24887 | 22565 | 4.3 | |