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. 2017 Jun 17;38(10):966–975. doi: 10.1093/carcin/bgx062

Figure 3.

Figure 3.

Over-expression of PSIP1/p75 promotes BC cell proliferation, migration and invasion. (A) Immunoblot showing the over-expression of transiently expressed T7-tagged p75 isoform in control and PSIP1-depleted M4 cells. (Ba–c) Anchorage-dependent colony formation assay performed in control and PSIP1-depleted M4 cells that are transiently transfected with empty vector or T7-p75. Graphs are derived from three independent experiments. (Ca–c) Migration assay performed in control and PSIP1-depleted M4 cells that are transiently transfected with empty vector or T7-p75. Migrated cells are stained and counted from three independent experiments. (D) Western blot showing the levels of stably overexpressed PSIP1/p75 in M2 cells. U2β”-snRNP is used as loading control in A and D. (Ea–b) Plastic colony formation assay performed in M2 cells after PSIP1/p75 over-expression. (Fa–b) Migration assay performed in PSIP1/p75 stably overexpressed M2 cells. Graph is derived from three independent experiments. Error bars in the graphs represent SEM. *P < 0.05 and ***P < 0.001.