Fig. 1.

Homologous site in human Oct4 shows similar transcriptional profile to mouse Oct4. Sequence analysis of human and mouse sequence around mapped mouse O-GlcNAc site (A). Western blot analysis to determine the presence of O-GlcNAc modification on Oct4 using O-GlcNAc specific antibody CTD110.6 on immunoprecipitated Oct4 protein expressed in HEK293T cells (B). Quantification of B using ImageJ software on 3 biological replicates. All samples were run on the same gel (see Supplementary Figure S1), lanes not pertaining to this figure were removed (C). The transcriptional activity of Oct4 determined by luciferase expression of mutant constructs in HEK293T cells showing fold change over WT with either monomer (6 W) promoter (D) or heterodimer (Oct/Sox) promoter (E). Experiments using the Oct/Sox promoter included expression of Sox2 in combination with Oct4. *P < 0.05. Student's t-test using 3 biological replicates.