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Overexpression of OGT alters transcriptional activity of Oct4. Western blot analysis to determine the presence of O-GlcNAc modification on immunoprecipitated Oct4 protein expressed in HEK293T cells (A). WT human Oct4 was expressed in HEK293T cells with either EGFP (control) or human OGT construct. Oct4 binds promoters in either monomer (6 W) (B) or heterodimer (Oct/Sox) (C and D) configurations. *P < 0.05. Student's t-test using at least 3 biological replicates.
