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Association with OGT alters transcriptional activation of Oct4 specific luciferase constructs in H9 hES cells. Luciferase activity with both promoters of endogenous Oct4 in H9 cells (A) or expressed WT-Oct4 in HEK293T cells (B and C) treated with or without 100 nM GNS. Luciferase activity of endogenous Oct4 in H9 cells expressing either EGFP (control), OGT or catalytically dead OGT (OGT H567A) (D). *P < 0.05. Student's t-test using at least 3 biological replicates.
