KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-CD31 (clone WM59) BV421 | Biolegend | Cat#303123; RRID: AB_2562179 |
| Anti-CD26 (clone BA5b) APC | Biolegend | Cat#302709; RRID: AB_10913814 |
| Anti-CD271 (clone ME20.4) PE | Biolegend | Cat#345105; RRID: AB_2282827 |
| Anti-CD326 (clone EBA-1) BB515 | BD | Cat#565398; RRID: AB_2728107 |
| Anti-CD45 (clone HI30) PerCP/Cy5.5 | Tonbo | Cat#65–0459; RRID: AB_2621897 |
| Anti-CD200 (clone OX-104) BV711 | Biolegend | Cat#329223; RRID: AB_2715823 |
| Anti-PDPN (clone NC-08) PE | Biolegend | Cat#337004; RRID: AB_1595554 |
| Rabbit anti-PSCA | Abcam | Cat#Ab64919; RRID: AB_1142338 |
| Donkey anti-rabbit IgG BV421 | Biolegend | Cat#406410; RRID: AB_10897810 |
| Chicken anti-KRT5 | Biolegend | Cat#905901; RRID: AB_2565054 |
| Anti-MYH11 (clone MYH11/923) | LS Bio | Cat#LS-C390741–100; RRID: AB_2728109 |
| Rabbit anti-Decorin | Sigma Aldrich | Cat# HPA003315; RRID: AB_1078639 |
| Rabbit anti-DHRS7 | Sigma Aldrich | Cat#HPA031121; RRID: AB_10600803 |
| Guinea pig anti-KRT8/18 | Fitzgerald | Cat#20R-CP004; RRID: AB_1284055 |
| Anti-KRT14(clone LL002) | Thermo Scientific | Cat#ms-115-p0; RRID: AB_63786 |
| Anti-KRT13 (clone EPR3671) | Abcam | Cat#ab92551; RRID: AB_2134681 |
| Anti-SCGB1A1 (clone 394324) | Novus Biologicals | Cat# MAB4218-SP; RRID: AB_2183286 |
| Anti-CHGA (clone CHGA(419)) | Sigma Aldrich | Cat#SAB4200728; RRID: AB_2728111 |
| Rabbit anti-SCG2 | Sigma Aldrich | Cat#HPA011893; RRID: AB_1856656 |
| Goat anti-chicken Alexa488 | Jackson Immunoresearch | Cat#103-545-155; RRID: AB_2337390 |
| Goat anti-mouse Alexa546 | Thermo Scientific | Cat#A11030; RRID: AB_2534089 |
| Goat anti-rabbit Alexa633 | Thermo Scientific | Cat#A21070; RRID: AB_2535731 |
| Goat anti-rabbit Alexa594 | Jackson Immunoresearch | Cat#111-516-045; RRID: AB_2728112 |
| Goat anti-guinea pig Alexa647 | Jackson Immunoresearch | Cat#106-605-003; RRID: AB_2337446 |
| Biological Samples | ||
| Donor 1, Age 31, prostate weight 23 g Used in Figures 1B, 2A–2C, and 5 | Southwest Transplant Alliance | N/A |
| Donor 2, Age 25, prostate weight 15 g Used in Figures 1B, 2A–2C, and 5 | Southwest Transplant Alliance | N/A |
| Donor 3, Age 29, prostate weight 20 g Used in Figures 1B, and 5 | Southwest Transplant Alliance | N/A |
| Donor 4, Age 18, prostate weight 16 g Used in Figures 3C and 4C | Southwest Transplant Alliance | N/A |
| Donor 5, Age 18, prostate weight 18 g Used in Figures 3C and 4C | Southwest Transplant Alliance | N/A |
| Donor 6, Age 19, prostate weight 17 g Used in Figures 3C and 4C | Southwest Transplant Alliance | N/A |
| Donor 7, Age 19, prostate weight 18 g Used in Figures 5C and 5D | Southwest Transplant Alliance | N/A |
| Donor 8, Age 24, prostate weight 15 g Used in Figures 5C and 5D | Southwest Transplant Alliance | N/A |
| Donor 9, Age 21, prostate weight 17 g Used in Figures 5C and 5D | Southwest Transplant Alliance | N/A |
| Donor 10, Age 18, prostate weight 18 g Used in Figures 5C and 5D | Southwest Transplant Alliance | N/A |
| Donor 11, Age 22, prostate weight 14 g Used in Figures 5C and 5D | Southwest Transplant Alliance | N/A |
| Deposited Data | ||
| Bulk and single cell sequencing of human prostate cells | GEO database | GSE120716 |
| All raw data | GUDMAP database | https://doi.org/10.25548/W-R8CM |
| Oligonucleotides | ||
| see Table S5 | This paper | N/A |
| Software and Algorithms | ||
| R code for scRNA-seq analysis | Strand Lab | https://git.biohpc.swmed.edu/StrandLab/sc-TissueMapper_Pr |