(A) Schematic of various RCAS vectors. Blue and red boxes represent components of C11orf95 and RELA coding sequences, respectively. Z, zinc finger (C2H2-like); RHD, Rel homology domain; AD, activation domain; orange box, 9aaTAD; H, HA tag. The phosphorylation and acetylation sites of the RELAFUS1 gene product corresponding to those of RELA are also described.
(B) Kaplan-Meier survival curves showing symptom-free survival of RCAS-RELAFUS1 (black line) and RELAFUS1-HA(red line), RELA-HA (blue line), or C11orf95-HA (green line) injection in Nestin (N)/tv-a (regular line) or N/tv-a;Ink4a-Arf−/− (dotted line) mice. Tumor formation was observed by injection of these RCAS viruses into neonatal pups brains. n.s., not significant.
(C) Summary of the RCAS vector injections in N/tv-a or N/tv-a;Ink4a-Arf−/−.