Fig. 4.
Activation of ephrin reverse signaling in does not increase cell death in FACS-sorted MEE cells. MEE cells grown in culture to 80% confluence were treated for 48 h with IgG Fc (−ve control), EphB2/Fc, or cisplatin (+ve control), then incubated with the nucleic acid dyes C12-resazurin (excitation at 488nm) and Sytox Red (at 633 nm) to label live and dead cells, respectively, and analyzed by flow cytometry. Live cells are shown in green (Q1). Dead cells are shown in red (Q4). Cell cycle-arrested cells are shown in pink (Q2) and cellular debris in blue (Q3). (A) 87.7% of IgG Fc-treated cell were alive and viable with only 0.6% intact cells dead. (B) EphB2/Fc treatment showed no difference in the number of dead cells compared to the negative control (86.5% viable and 0.4% dead cells). (C) Cisplatin treatment caused cycle arrest in 19.1% and death in 30.2% of total cells.