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. Author manuscript; available in PMC: 2019 Mar 11.
Published in final edited form as: J Cell Physiol. 2015 Dec;230(12):2961–2972. doi: 10.1002/jcp.25025

Fig. 5.

Fig. 5

Ephrin reverse signaling causes EMT-like marker changes in mouse palatal MEE cells. Embryonic mouse MEE cells were cultured for 48 h in either IgG Fc or EphB2/Fc protein at 5 ng/mL, then fixed and processed for immunofluorescent detection of epithelial or mesenchymal markers. (A) Expression of the epithelia-specific cell junction markers E-cadherin, demosplakin, and plakoglobin (green) virtually disappeared after 48 h of EphB2/Fc treatment. (B) Expression of the mesenchymal markers fibronectin (green) and vimentin (red) increased dramatically after 48 h of EphB2/Fc exposure while expression of epithelia-associated proteins E-cadherin (red) and Z01 (green) essentially disappeared.