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. 2019 Mar 11;14(3):e0213673. doi: 10.1371/journal.pone.0213673

Fig 3. Proliferating cell nuclear antigen (PCNA) immunohistochemistry and mRNA expression in the cerebrum 1 to 14 days after injury.

Fig 3

(A) PCNA immunostained cells could be observed in the ipsilateral hemicerebrum at 1, 4, 7, and 14 days after injury in injury groups. Long scale = 50 μm, short scale = 12.5 μm. (B-D) Double immunohistochemistry of the ipsilateral hemicerebrum at 4 days after injury for co-localization of (B) PCNA, NeuN, DAPI (4',6-diamidino-2-phenylindole), and merge; (C) PCNA, ionized calcium binding adaptor molecule 1 (Iba 1), DAPI, and merge; and (D) PCNA, glial fibrillary acidic protein (GFAP), DAPI, and merge. Scale = 30 μm. (E) Graph of the numbers of PCNA immunostained cells in (A). (F-H) Pcna mRNA expression in the ipsilateral hemicerebrum 1 to 14 days after injury. Pcna mRNA expressions were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (Gapdh) (F), hypoxanthine guanine phosphoribosyl transferase (Hprt) (G), and peptidylprolyl isomerase A (Ppia) (H) (*p < 0.05, n = 5 for control group, n = 5 for the sham group, n = 5 for the injury group).