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Flow cytometry results of L. major promastigotes exposed to different peptides. Effective peptides affected L. major through necrosis. No signs of apoptosis event were detected. (A) Untreated parasite stained with Annexin/PI, (B) Parasite treated with 0.5% Triton x-100 as positive control for PI. (C) Brevinin 2R, (D) L- Brevinin 2R, (E) CLIP and (F) L- CLIP treated parasite. In each pair of graphs, the left represents SSC vs FSC and the right graph indicates PI vs Annexin value.
