Fig 9.
(A) Footpad swelling in weeks after challenge until the end of treatment. Swelling was calculated according to thickness and width of the footpad. Infected foot size was subtracted from intact footpad. (B) Footpad swelling in experimental groups at 5th week after challenge. (C) Real-time PCR assay for parasite burden determination in the lymph node of experimental animals. Peptide (L- Brevinin 2R) with or without CpG motif was able to control the parasite load in the lymph nodes adjacent to the infected footpad. (D) Normalized data provided from GAPDH house-keeping gene Real- time PCR assay in parallel with parasite burden in lymph node samples. *, ** and *** = P value < 0.05, < 0.01 and < 0.001 respectively.