Table 3.
Plaque Reduction Neutralization Test Results for Representative Samples With CHIKV IgG Antibodies Determined by ELISA
| Probit Titer PRNT 80 | ||||
|---|---|---|---|---|
| Sample | District | IgG ELISA OD | CHIKV | ONNV |
| 102068 | Kampala | 0.39 | 144 | 2330 |
| 102004 | Kampala | 0.70 | 155 | 4058 |
| 101608 | Kampala | 3.50 | 464 | 5814 |
| 103997 | Mbale | 0.28 | 89 | 1093 |
| 103998 | Mbale | 0.76 | 289 | 6321 |
| 104004 | Mbale | 2.86 | 1211 | 13491 |
| 105225 | Mbarara | 0.36 | 33 | 1100 |
| 105516 | Mbarara | 0.88 | 94 | 2486 |
| 105212 | Mbarara | 1.67 | 96 | 4328 |
| 106359 | Fort Portal | 0.50 | 203 | 4209 |
| 106710 | Fort Portal | 0.95 | 122 | 1297 |
| 106715 | Fort Portal | 1.65 | 114 | 2402 |
| 111146 | Gulu | 0.45 | 39 | 1556 |
| 107180 | Gulu | 0.69 | 112 | 2128 |
| 107077 | Gulu | 1.39 | 363 | 4239 |
Each sample was tested for the ability to neutralize 80% CHIKV and ONNV. An 80% probit neutralization titer was calculated for each virus.
Abbreviations: CHIKV, chikungunya virus; ELISA, enzyme-linked immunosorbent assay; IgG, immunoglobulin G; OD, optical density; ONNV, o’nyong-nyong virus; PRNT, plaque reduction neutralization test.