Fig 5. Py17XNL-specific IgG binds weakly to the surface of pRBC in vivo.

(A) Schematic showing that mice (n = 5/group) were injected with immune or non-immune control serum 24h prior to infection with CTFR⁺ Py17XNL-infected pRBCs, with surface deposition of IgG on pRBC assessed 1h and 4h afterwards. (B) Representative FACS plots showing detection of in vivo-deposited mouse IgG on the surface of CTFR⁺ RBC (after 1h & 4h in vivo exposure), with positive controls stained in vitro with RBC-specific mouse IgG (34-3c). Data are representative of two independent experiments showing similar results. Statistics: Mann-Whitney t-test, **P < 0.01. (C-D).