Fig. (2).

Approximate timeline for processing and handling of blood collected from participants in the randomized arm of the PLCO Trial. Blood was drawn according to the protocols at the PLCO screening centers. Yellow top tubes were drawn at the T3 visits only. For the red, blue-top, green, and lavender top tubes, the time between blood draw through to centrifugation, isolation of components, aliquoting into cryovials and placing the cryovials in the freezer was 2 hours. Within a month after freezing the cryovials, they were shipped to the biorepository for long term storage. Cryopreserved whole blood was also shipped to the biorepository for long term storage.