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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Hepatology. 2019 Feb 8;69(3):1046–1063. doi: 10.1002/hep.30300

FIG. 5.

FIG. 5.

DDX5 promotes autophagy by interfering with the binding of p62 to TRAF6. (A) Mapping of p62 regions involved in DDX5 binding. Top: different truncated mutants of p62. Bottom: HEK 293T cells were co-transfected with indicated constructs of p62-Myc and DDX5-Flag. Cell extracts were IP with anti-Flag antibody. *, heavy chain. (B) HepG2 cells were transfected with vector or DDX5-Flag. The cell extracts were coimmunoprecipitated (IP) with anti-p62 antibody and blotted with anti-TRAF6 antibody. (C) HEK293T cells were co-transfected with p62-HA, TRAF6-Myc, and DDX5-Flag. Cell extracts were IP using anti-HA antibody and blotted with anti-Myc antibody. (D) HepG2 cells stably expressing vector or DDX5 were transfected with control-siRNA or TRAF6-siRNA. Cell lysates were collected, and the autophagy-related proteins were analyzed. Data are representative immunoblots of three independent assays. The ratio of LC3B-II/LC3B-I was presented as mean ± SEM (n = 3). NS: nonsignificant.