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. 2019 Feb 18;54(4):1195–1208. doi: 10.3892/ijo.2019.4717

Figure 3.

Figure 3

Effects of COPB2 gene knockdown on cell proliferation, as determined by MTT and BrdU assays. (A) MTT assay was conducted and absorbance at 490 nm was compared, indicating the number of active BGC-823 cells in the Lv-shCOPB2 and Lv-shCtrl groups. The proliferation rate of BGC-823 cells was significantly inhibited in the Lv-shCOPB2 infection group at 4 and 5 days post-infection (P<0.05), indicating that the COPB2 gene may be significantly associated with the proliferation ability of BGC-823 cells. (B) BrdU incorporation assay was conducted and OD was detected. Cell proliferation in the Lv-shCOPB2 group was significantly reduced compared with in the Lv-shCtrl group 4 days post-transduction. ***P<0.01, compared with the Lv-shCtrl group. BrdU, 5-bromo-2-deoxyuridine; COPB2, coatomer protein complex subunit β2; Lv, lentivirus; OD, optical density; sh, short hairpin RNA.