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. 2019 Feb 22;54(4):1168–1182. doi: 10.3892/ijo.2019.4723

Figure 3.

Figure 3

Expression of mutated p53 influences the migration and invasiveness of cancer cells. (A) MDA-MB-231 and H1299 cells with indicated p53 variants were seeded into wells with ORIS stoppers and, where applicable, induced with 30 ng/ml DOX. After 48 h (MDA-MB-231) or 24 h (H1299) the stoppers were removed (t=0). The relative migration speed for each cell type was calculated by dividing the speed of the DOX-induced cells by the speed of non-induced cells and normalizing to the value obtained for the SH or TR cells, respectively. The migration speed with and without Matrigel was measured by xCELLigence for (B) MDA-MB-231 and (C) H1299 cells expressing the indicated p53 mutants. The migration speed values are normalized to the speed of MDA-MB-231-SH or H1299-TR cells without Matrigel. (D) The invasiveness (relative migration with vs. without Matrigel) was measured for MDA-MB-231 or H1299 cells expressing various p53 mutants at 16 or 48 h, respectively. The data are presented as the mean ± standard error of the mean. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. p53, tumor suppressor p53; DOX, doxycycline; ns, not significant; TR, cells with Tet repressor only; SH, cells with silenced endogenous p53; TA, transactivatory domain disruption (L22S/W23Q) in addition to indicated mutation (R248Q or D281G).