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. 2019 Feb 22;54(4):1168–1182. doi: 10.3892/ijo.2019.4723

Figure 4.

Figure 4

Collagen I local infiltration and invasiveness are altered by the expression of mutated p53. (A) Microspheres were prepared from MDA or H cells conditionally expressing the indicated p53 variants and induced or not induced with 30 ng/ml DOX. The spheres were embedded in 0.5 mg/ml collagen I matrix and imaged at 0, 1 and 2 dpe. Changes in the main micro-sphere circularity and the NOC separated from the main microsphere were calculated in relation to the microspheres composed of cells not induced with DOX. A decrease in circularity reflects an irregular microsphere shape resulting from increased local invasiveness. (B) A representative set of microsphere images. Depicted are microspheres composed of MDA-MB-231 cells expressing the indicated p53 variants. The cells were DOX-induced 48 h prior to embedding. The images were captured directly post-embedding and at 48 h. Scale bar, 1 mm. (C) Magnification of the 2 dpe images. Scale bar, 0.5 mm. The data are presented as the mean ± standard error of the mean. *P<0.05 and **P<0.01, respectively. p53, tumor suppressor p53; MDA, MDA-MB-231; H, H1299; DOX, doxycycline; dpe, days post-embedding; NOC, number of cells; ns, not significant; TR, cells with Tet repressor only; SH, cells with silenced endogenous p53; TA, transactivatory domain disruption (L22S/W23Q) in addition to R248Q mutation.