Figure 2.

The effect of vitamin D derivatives on the proliferation of human melanoma A375 cells that were treated with cisplatin. Melanoma A375 cells were treated with serial dilutions of cisplatin (0.019-300 µM) in combination with 10 nM (A) 1α,25(OH)₂D3, (B) 20(OH)D3, (C) 21(OH)pD or (D) calcipotriol for 48 h. The results are representative of three experiments (n=6). The same control data is plotted in each graph. ^*P<0.05 between the two treatments at each cisplatin concentration, using one-way analysis of variance followed by Tukey’s multiple comparison test. 1α,25(OH)₂D3, 1α, 25-dihydroxyvitamin D3; 20(OH)D3, 20S-hydroxyvitamin D3; 21(OH)pD, 21-hydroxypregnacalciferol.